FelixTRIzol reagent is a complete ready-to-use reagent that can isolate high-quality total RNA from various biological samples or simultaneously separate RNA, DNA and proteins. This single-phase solution of phenol and Chemicalbook isothiocyanate guanidine can separate the individual components of RNA, DNA and proteins from cell and tissue samples derived from humans, animals, plants, yeast, or bacteria within one hour.
The main components include phenol, guanidine isothiocyanate (GITC), and chloroform, etc. Phenol can damage the cell membrane, releasing the contents inside the cell; GITC can inhibit the activity of nucleases and prevent RNA degradation; chloroform is used to separate the organic phase from the aqueous phase.
When using Trizol reagent to extract total RNA, it can maintain the integrity of the RNA, and it can process a large number of different samples simultaneously while obtaining high-quality total RNA.
